TABLE 3.
Relative phzM transcription levels in wild-type strain M18, lasI::Gm mutant M18LSG, complemented M18LSG, ptsP::Gm mutant M18PP, and complemented M18PP determined by qRT-PCR during early and late exponential phase
| Strain | Early log phase
|
Late log phase
|
||||||
|---|---|---|---|---|---|---|---|---|
| CT,rpoDa | CT,phzMa | ΔCTb | Mc | CT,rpoDa | CT,phzMa | ΔCTb | Mc | |
| M18 | 20.05 ± 0.5 | 23.94 ± 0.1 | 3.89 ± 0.1 | 1.00 | 20.14 ± 0.01 | 22.09 ± 0.2 | 1.71 ± 0.05 | 1.00 |
| M18LSG | 19.78 ± 0.09 | 25.48 ± 0.12 | 5.70 ± 0.1 | 0.28 | 20.28 ± 0.03 | 23.65 ± 0.05 | 3.37 ± 0.04 | 0.37 |
| Complemented M18LSG | 20.07 ± 0.02 | 23.96 ± 0.07 | 3.89 ± 0.02 | 1.01 | 20.10 ± 0.02 | 21.81 ± 0.03 | 1.702 ± 0.03 | 1.19 |
| M18PP | 20.06 ± 0.27 | 21.05 ± 0.89 | 0.99 ± 0.1 | 4.26 | 19.76 ± 0.08 | 19.20 ± 0.66 | -0.56 ± 0.04 | 4.66 |
| Complemented M18PP | 19.60 ± 0.03 | 21.84 ± 0.26 | 2.24 ± 0.1 | 1.79 | 19.68 ± 0.09 | 20.67 ± 0.70 | 0.99 ± 0.07 | 1.59 |
a
Values were measured during early exponential phase at an OD600 of 2.0 to 2.4 and during late exponential phase at an OD600 of 5.0 to 6.0. All values are means ± standard deviations of three independent experiments.
b
ΔCT = mean CT, phzM − mean CT, rpoD.
c
M = 2−ΔΔCTphzM normalized amount of cDNA from the phzM gene in different strains relative to that in wild-type Pseudomonas sp. strain M18; ΔΔCT = mean ΔCT − mean ΔCT, M18.