TABLE 1.
Primers used for the transcriptomic study of Y. lipolytica 1E07 genes
| Primer | Accession no. | Sequence (5′-3′) | Putative functionb |
|---|---|---|---|
| GAP1-R | YALI0B16522g | CGACCACAGCAATGACTTTAATA | Amino acid transporter |
| GAP1-F | AACTACTGGAATGAAGCTAACG | ||
| ARO8-R | YALI0E20977g | GGCTCCGACCCAGTTGT | Aromatic amino acid aminotransferase |
| ARO8-F | TTCTCCTCCGCCATCGAGTG | ||
| BAT1-R | YALI0D01265g | GTTGGCTCCCAGCTTCTTGT | Branched-chain amino acid aminotransferase |
| BAT1-F | CTCTCGGCGTCGGAACC | ||
| BAT2-R | YALI0F19910g | TCCAACGGCCTTGGAGTTCT | Branched-chain amino acid aminotransferase |
| BAT2-F | CCTCAAGCTCTACTGCTCCGA | ||
| JEN1-R | YALI0D20108g | TTAATGTGAGCGTCACAGATATCAC | Organic acid transporter |
| JEN1-F | AGCTCCAGCACAATAAATAGAACAC | ||
| GHD2-R | YALI0E09603g | CTTGAGGAGCAAATCAATGACC | Glutamate dehydrogenase |
| GDH2-F | TCCATGTTCGACGAGAACTAC | ||
| GHD3-R | YALI0F17820g | CTTAGAGTCGGACATGGAGACAAC | Glutamate dehydrogenase |
| GDH3-F | TACGTTGAGAAGATGATTGAGTACG | ||
| GND1-R | YALI0B15598g | GATGTCCTGGAAAATCTTCTTAATG | Pentose pathway |
| GND1-F | GATATCATCATTGACGGTGGTAACT | ||
| PGI1-R | YALI0F07711g | GGTTCTCTGTGAAGTTGATCTTGTC | Glycolysis |
| PGI1-F | CTTTGATGACTCCAAGATTCTGTTT | ||
| PYC1-R | YALI0C24101g | CAGAGATAACCATCTCCATCTTCAT | Pyruvate carboxylase |
| PYC1-F | GAAAGATTTCTGTTGAGGACAAGAA | ||
| CHA1-R | YALI0B16214g | TTTCCTCCAGAAGAAGAAAAGAAGT | l-Serine/l-threonine deaminase |
| CHA1-F | TGCTTCTCAAATACGAAACTACACA | ||
| HXT2-R | YALI0F19184g | ATAGAAAAAGTAGTTGGCACCACAG | High-affinity hexose transporter |
| HXT2-F | GAACTCAAGGCTATTGAGAACTCTG | ||
| DLD1-R | YALI0E03212g | AAACGTATTCCTCACCGATAG | Lactate oxidoreductase |
| DLD1-F | TGGCCCTTAAGAAGGAAGAT | ||
| KAD-R | YALI0D08690g | CTACTACTCGGTAAGTAGGCATGGA | 2-Oxoisovalerate dehydrogenase |
| KAD-F | AAAACATGTCCATAAGACCCAGTT | ||
| PDA1-R | YALI0F20702g | TAGATATCCTCAAACAGAACCTTGG | Pyruvate dehydrogenase |
| PDA1-F | AACGATCCTATTTCTGGTCTCAAG | ||
| PDB1-R | YALI0E27005g | AGTCTTCTTGATGGAGTTGAAAATG | Pyruvate dehydrogenase |
| PDB1-F | TAAGGATATCACTCTTGTCGGTCAC | ||
| CYB2-R | YALI0E21307g | TGCATCCACTGAGTCTGTTT | LDH |
| CYB2-F | TACATCACCGCTACAGCTCTA | ||
| Act21r | YALI0D08272ga | GGCCAGCCATATCGAGTCGCA | Gene encoding actin |
| Act20 | TCCAGGCCGTCCTCTCCC |
a
Data from reference 6.
b
Annotations from Génolevures (http://cbi.labri.fr/Genolevures/).