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Assessment of intracellular Ca2+ handling and cardiomyocyte contractility. (A-C) Protein expression of calcium cycling proteins, SERCA2a, PLN, p-PLN and NCX1. (D) Percentage shortening and (E) intracellular calcium levels in freshly isolated cardiomyocyte. Data are expressed as mean ± SEM from 4-5 hearts for each group. *p < 0.05 vs LFWT.
