Figure 4.

(A–C) Expression of three miRNAs (miR-101, miR-202-5p, and miR-148a) was assessed during oogenesis and in eggs. Stages I and II, III and IV, and V and VI were pooled. One hundred and fifty oocytes were used for each experiment. (D–F) The expression of the same three miRNAs was assessed in early stage oocytes (stages I and II) and a number of adult somatic tissues using Northern blotting. Ten micrograms of total RNA were used for each lane. EDC was used to crosslink small RNAs to the membrane prior to hybridization. 5S rRNA is shown as a loading control.