Detection of exonic, intronic, and antisense tags in the Tag-seq and LongSAGE hESC replicates. Tag sequences from the Tag-seq technical replicate, the in silico derived sub_Tag-seq, and the LongSAGE replicate were mapped to the introns, exons, and antisense strands of Ensembl genes. The proportions of distinct tag sequences (A) and tag abundance (B) are reported relative to all mapped quality-filtered tags. Average tag counts (±SD) are reported for all tag sequences found in common between the three libraries (C).