Figure 2.

Sanshools, 6-shogaol, 6-paradol and linalool activate capsaicin- and cinnamaldehyde-sensitive dorsal root ganglia neurons. Each panel shows representative changes in [Ca²⁺]_i of three neurons to capsaicin (Cap; TRPV1), cinnamaldehyde (Cinna; TRPA1) and menthol (TRPM8). Compounds were applied successively for 5 s, as shown by horizontal bars. Between stimulations neurons were perfused with supplemented HBSS. Maximal concentrations were used in these experiments. (A) 500 µM α-SOH, 100 µM Cinna, 1 µM Cap, 500 µM Menthol; (B) 500 µM I, 500 µM IV, 100 µM Cinna, 1 µM Cap, 500 µM Menthol; (C) 500 µM II, 500 µM III, 100 µM Cinna, 1 µM Cap, 500 µM Menthol; (D) 500 µM 6-shogaol, 100 µM Cinna, 1 µM Cap, 500 µM Menthol; (E) 500 µM 6-paradol, 100 µM Cinna, 1 µM Cap, 500 µM Menthol; (F) 500 µM linalool, 100 µM Cinna, 500 µM Menthol, 1 µM Cap. α-SOH, hydroxy-α-sanshool; TRPA1, transient receptor potential ankyrin 1; TRPM8, transient receptor potential melastatin 8; TRPV1, transient receptor potential vanilloid 1.