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. 2009 Jul 8;157(8):1398–1409. doi: 10.1111/j.1476-5381.2009.00307.x

Figure 5.

Figure 5

Targets of the N-terminal reactive cysteines in TRPA1. Voltage changes of HEK293 cells loaded with Red dye expressed as fluorescence intensity (FI) when stimulated with maximal concentrations of the tested compounds. Cells were transiently transfected with wild-type TRPA1 and TRPA1-3C and stimulated with (A) 150 µM cinnamaldehyde (Cinna), (B) 100 µM 2-APB. (C) 500 µM α-SOH. (D) Recapitulates peak responses of the cells to 150 µM Cinna, 100 µM 2-APB, 500 µM α-SOH, 500 µM II, 500 µM III, 500 µM IV, 100 µM 6-shogaol, 500 µM 6-paradol and 500 µM linalool. *P < 0.05 unpaired Student's t-test. + indicates the compounds that formed adducts with GSH. Means ± SEM (n= 4–5). 2-APB, 2-aminoethyl diphenyl borate; GSH, glutathione; α-SOH, hydroxy-α-sanshool; TRPA1, transient receptor potential ankyrin 1; TRPM8, transient receptor potential melastatin 8; TRPV1, transient receptor potential vanilloid 1.