Figure 6.

Compounds activate TRPV1 via non-covalent gating. Voltage changes of HEK293 cells loaded with Red dye expressed as a fluorescence intensity (FI) when stimulated with saturating concentrations of compounds. Cells were transiently transfected with wild-type TRPV1 and TRPV1-C158A and typical responses are shown for (A) 1 µM capsaicin (Cap), (B) 2 mM MTSEA, (C) 500 µM α-SOH. Means ± SEM (n= 4–5). MTSEA, 2-aminoethyl methanethiosulphonate hydrobromide; TRPV1, transient receptor potential vanilloid 1.