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. 2009 Jul 7;157(8):1354–1367. doi: 10.1111/j.1476-5381.2009.00261.x

Figure 5.

Figure 5

Localization of the curcumin response element(s) in the ldlr promoter. Semi-confluent hepatic stellate cells (HSCs) in six-well plates were transiently transfected with luciferase reporter plasmids. After recovery, cells were treated with or without curcumin as shown for 24 h. Luciferase assays were performed. Luciferase activities were expressed as relative units after β-galactosidase normalization (means ± SD; n≥ 6). The insets denoted the pLDLR-Luc constructs in use and the application of curcumin to the system. (A) Luciferase assays of cells transfected with the ldlr promoter luciferase reporter pLDLR-Luc with various lengths of the 5′-flanking promoter region of the ldlr promoter. *P < 0.05 versus cells transfected with pLDLR-Luc with the −1500 bp promoter region (p-1500-Luc); ‡P < 0.05 versus cells transfected with pLDLR-Luc with the −335 bp promoter region (p-335-Luc). (B) Luciferase assays of cells transfected with pLDLR-wt-Luc or pLDLR-mut-Luc, containing wild-type sterol regulatory element (SRE) or mutated SRE respectively. *P < 0.05 versus cells without curcumin treatment.