Skip to main content
. 2009 Aug;157(8):1463–1473. doi: 10.1111/j.1476-5381.2009.00314.x

Figure 4.

Figure 4

(A) Isometric force recordings showing the relaxations evoked by electrical field stimulation (EFS, 1 ms duration, 1–16 Hz, 20 s trains) and exogenous adenosine 5′-triphosphate (ATP) (1 µM–0.3 mM), in the absence or presence of 8-(p-sulphophenyl)theophylline (8-SPT) (100 µM), on 1 µM phenylephrine (PhE)-precontracted pig urinary bladder neck strips treated with guanethidine (10 µM), atropine (0.1 µM) and NG-nitro-L-arginine (100 µM). Vertical bar shows tension in grams, and horizontal bar shows time in minutes. (B,C) Frequency-response and (D,E) log concentration-response relaxation curves to EFS and exogenous ATP, respectively, in control conditions and in the presence of 8-SPT (100 µM) (B, D) and 8-SPT plus 2′-deoxy-N6-methyladenosine 3′,5′-bisphosphate tetrasodium (MRS2179) (10 µM) (C,E). Results are expressed as a percentage of the PhE-induced contraction and represent mean ± SEM of six to seven preparations. *P < 0.05 versus control (paired t-test).