Table 5.
Effects of intact urothelium and of blockade of neuronal voltage-gated Na+ channels with TTX on relaxations evoked by ATP, ADP and adenosine
|
ATP |
|||
|---|---|---|---|
| n | pEC50 | R (%) | |
| Urothelium denuded | 7 | 3.7 ± 0.2 | 92.3 ± 4.1 |
| Urothelium intact | 7 | 3.4 ± 0.2* | 79.5 ± 9.7 |
| Control | 7 | 3.9 ± 0.1 | 91.7 ± 4.4 |
| TTX (1 µM) | 7 | 3.9 ± 0.1 | 88.8 ± 5.1 |
| ADP | |||
| Urothelium denuded | 6 | 4.0 ± 0.1 | 97.9 ± 2.1 |
| Urothelium intact | 6 | 3.6 ± 0.1* | 89.1 ± 8.7 |
| Control | 6 | 4.0 ± 0.1 | 90.0 ± 4.7 |
| TTX (1 µM) | 6 | 4.1 ± 0.1 | 88.9 ± 4.5 |
| Adenosine | |||
| Urothelium denuded | 7 | 4.1 ± 0.1 | 95.5 ± 4.1 |
| Urothelium intact | 7 | 3.8 ± 0.1* | 85.6 ± 5.5 |
| Control | 7 | 4.0 ± 0.1 | 93.0 ± 5.0 |
| TTX (1 µM) | 7 | 4.0 ± 0.1 | 92.8 ± 4.7 |
Results are expressed as mean ± SEM of n experiments.
*
P < 0.05 versus control (unpaired and paired t-tests in case of urothelium and TTX respectively).
ADP, adenosine 5′-diphosphate; ATP, adenosine 5′-triphosphate; pEC50 = −log EC50, where EC50 is the concentration of agonist producing 50% relaxation of phenylephrine (PhE)-induced contraction; R is the relaxation, expressed as a percentage of the PhE-induced contraction, evoked at the highest concentration of agonist used: ATP, 1 mM; ADP, 1 mM; and adenosine, 1 mM; TTX, tetrodotoxin.