Figure 4.

Expression of mRNA and Western blot analysis for urocortin and corticotrophin-releasing factor (CRF)_1/2 receptors in femoral arteries from normal, sham operated and thromboangiitis obliterans (TAO) model rats. In (A), a representative record of the expression of mRNA is shown (Ucn, urocortin; CRF-R1, CRF₁-receptor; CRF-R1α, CRF_1α-receptor; CRF-R2, CRF₂-receptor) with GAPDH as an internal control. Summary data are shown in (B); data are presented as means ± SEM (n= 8). *P < 0.05, versus sham operated group. In (C), a representative Western blot for CRF₁-receptors and CRF₂-receptors is shown (β-actin as control) with summary data in (D); data presented as means ± SEM (n= 8). *P < 0.05, versus sham operated group.