Table 1.
Summary of the RT-PCR primer sequences used to amplify GAPDH, urocortin, CRF1-receptors, CRF2-receptors, COX-2 and ICAM-1 from rat tissues
| Sequences | Product size (bp) | Annealing T(C) | ||
|---|---|---|---|---|
| GAPDH | Sense | TCCCAGAGCTGAACGGGAAGCTCACTG | 339 | 68.1 |
| Antisense | TGGAGGCCATGTAGGCCATGAGGTCCA | |||
| Urocortin | Sense | GCTACGCTCCTGGTAGCGTTGCTGCTTCTG | 356 | 68.1 |
| Antisense | GCCGATCACTTGCCCACCGAGTCGAATATG | |||
| CRF1 | Sense | AAGGCGGGATCCAGGCAGTAGAGA | 508 | 60.8 |
| Antisense | TCCCGGTAGCCATTGTTTGTCGTG | |||
| CRF1α | Sense | TCCTACGCAACGCCAC | 147 | 58.8 |
| Antisense | AGCAGCCCTCACCGAAC | |||
| CRF2 | Sense | CTGGTGGCTGCTTTCCTGCTTTTC | 425 | 58.1 |
| Antisense | ATGGGGCCCTGGTAGATGTAGTCC | |||
| COX-2 | Sense | TTCACCAGACAGATTGCTGGC | 530 | 63.5 |
| Antisense | AGTCTGGAGTGGGAGGCACTTG | |||
| ICAM-1 | Sense | AGAAGGACTGCTTGGGGAA | 332 | 58.1 |
| Antisense | CCTCTGGCGGTAATAGGTG | |||
CRF, corticotrophin-releasing factor; COX, cyclooxygenase; ICAM-1, intercellular adhesion molecule-1.