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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Hepatol. 2009 May 3;51(1):139–148. doi: 10.1016/j.jhep.2009.03.024

Figure 5. Phagocytosis of AB Induces ROS Production and Akt Phosphorylation.

Figure 5

(A) LX-2 cells were incubated with AB for 6 hours and cytochrome c assay was used to evaluate ROS production. Phagocytosis of AB induced 2.2±0.4-fold of superoxide activity compared to control. N=4, *p<0.05. (B) LX-2 cells were exposed to AB with or without apocynin (NADPH oxidase inhibitor), AG490 or LY294002, and the cell lysates were collected for immunoblotting using antibodies to phosho-Akt and total Akt. AB transiently induced Akt phosphorylation, which was blocked by apocynin and LY294002 respectively, but not AG490.