Figure 6. Phagocytosis of AB Induces NF-κB activation.
LX-2 cells were incubated in a serum free medium then exposed to AB with or without prior preincubation with CAPE (1ng/ml, 14 hours), LY294002 (600 nM, 1 hour), AG490 (50μM, 1 hour), or apocynin (100μM, 1 hour). Engulfment of the AB by LX-2 cells induced a significant activation of NF-κB (3.65±0.55-fold), and this was inhibited by CAPE, PI3K inhibition, and to a lesser extent by JAK and NADPH oxidase inhibition. Densitometry shows data from 3 experiments. *p<0.005