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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Exp Neurol. 2008 Sep 24;215(1):5–19. doi: 10.1016/j.expneurol.2008.09.003

Figure 10.

Figure 10

Human macrophages express significantly greater amounts of CD200R mRNA and protein than human microglia. A: Characterization of custom made antibody to CD200R (A2524) using HEK cell line transfected with control vector (Con) or CD200R expression plasmids. Antibody recognized band only in CD200R plasmid transfected cells. Molecular weight of CD200R varied between different HEK isolates. B: CD200R mRNA is expressed by microglia (MG), macrophages (MAC) and differentiated SH-SY5Y neuronal cells. Expression of CD200R mRNA by microglia is noticeably less than by macrophages. C: Detection of CD200R protein with molecular weight of 66-68 kD by western blot in extracts of human microglia (MG), macrophages (MAC), and SH-SY5Y neuronal cells, and in CD200R transfected HEK cells (CD200R-HEK - at 1% of protein level compared to other cells). Protein bands were detected with custom antibody to CD200R (A2524). D: Quantitative measurements of western immunoblots showed that macrophages express significantly greater amounts of CD200R protein than human microglia.