Table 2. Amplicon sizes and optimum temperatures for DHPLC analysis of all individual WDR36 exons.
|
Exon |
Amplicon size (bp) |
Temperatures (°C) |
| 1 |
724 |
62.1/63.0/63.6 |
| 2 |
500 |
53.8 |
| 3 |
414 |
57.1 |
| 4 |
402 |
53.8/55.1 |
| 5 |
469 |
53.5/54.5/56.5 |
| 6 |
465 |
54.0/55.0 |
| 7 |
396 |
55.5/55.9 |
| 8 |
361 |
57.8/58.3 |
| 9 |
392 |
57.0/57.4 |
| 10 |
362 |
56.5/57.0 |
| 11 |
450 |
54.5/55.5/56.0 |
| 12 |
531 |
55.9/56.5/57.2 |
| 13 |
450 |
55.5/56.5 |
| 14 |
376 |
55.4/56.5/57.5 |
| 15 |
400 |
57.4/58.7 |
| 16 |
534 |
56.1/57.3 |
| 17 |
282 |
55.2/57.6 |
| 18 |
695 |
54.1/56.3 |
| 19 |
438 |
53.9/54.9 |
| 20 |
485 |
56.0 |
| 21 |
301 |
54.2/55.1 |
| 22 |
516 |
58.0 |
| 23 | 643 | 54.3/54.9 |
Temperatures used for denaturing high performance liquid chromatography. In the table, bp indicates base pairs.