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Synthetic strategy toward homogeneous erythropoietin.
(a) Fragment condensation between G77 and Q78; (b) Native chemical ligation between G28 and C29. The ribbon diagram of the tertiary structure of human EPO protein is shown in green. N-linked glycosylation sites, N24, N38 and N83 are shown in red and O-linked glycosylation site, S126, in blue.
