The fragment condensation strategy for the synthesis of EPO(78-113) glycopeptide.a
aThe protected glycans are shown in star shape. Tmb is an abbreviation for 4,5,6-trimethoxy-2-methylthiobenzyl. The dipeptides used in SPPS are underlined. The protected amino acid residues are shown in italic. Reagents and conditions: (a) HATU, DIEA, DMSO, 29%; (b) TCEP•HCl, HOOBt, DIEA, DMSO, 33%; (c) AgCl, HOOBt, DIEA, DMSO, 30%.