Table 2.
Structure and properties of the galanin analogs. All analogs are amidated at the C-terminus.
| Analog | Structure | HPLC retention timea | α-helixb | Mass spec. (MALDI, MH+) Calcd/found |
|---|---|---|---|---|
| Gal-(K)4c | (Sar)WTLNSAGYLLGPKKKK | 15.13 ± 0.42 | 14% | 1873.09/1873.14 |
| Gal-B2-C8 | (Sar)WTLNSAGYLLGPKK(Lys-octanoyl)K | 20.13 ± 0.17 | 9% | 2001.21/2001.17 |
| Gal-B2-C10 | (Sar)WTLNSAGYLLGPKK(Lys-decanoyl)K | 21.49 ± 0.05 | 6% | 2029.24/2029.45 |
| Gal-B2-C12 | (Sar)WTLNSAGYLLGPKK(Lys-lauroyl)K | 22.51 ± 0.03 | 16% | 2057.28/2057.26 |
| Gal-B2-C14 | (Sar)WTLNSAGYLLGPKK(Lys-myristoyl)K | 23.76 ± 0.18 | 16% | 2085.31/2085.33 |
| Gal-B2c | (Sar)WTLNSAGYLLGPKK(Lys-palmitoyl)K | 25.84 ± 0.10 | 23% | 2112.31/2112.33 |
| Gal-B2-C18 | (Sar)WTLNSAGYLLGPKK(Lys-stearoyl)K | 26.73 ± 0.18 | 5% | 2141.33/2141.48 |
| Gal-B2-MPEG4 | (Sar)WTLNSAGYLLGPKK(Lys-MPEG4)K | 17.52 ± 0.03 | 18% | 2123.23/2123.29 |
a
A linear gradient of water/90% acetonitrile (buffered with 0.1% TFA) on a Vydac diphenyl column, starting from 20% acetonitrile to 100% acetonitrile in 40 min.
b
Determined in the presence of 50% v/v 2,2,2-trifluoroethanol.
c
Data from 22.