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. Author manuscript; available in PMC: 2010 Apr 22.
Published in final edited form as: J Am Chem Soc. 2009 Apr 22;131(15):5444–5459. doi: 10.1021/ja806534r

Figure 5.

Figure 5

Hydroxyl loss from the charge reduced cation radical of 3-nitrobenzylcysteine containing peptide. a) ECD, b) IRMPD/ECD and c) MALDI-TOF MS of 3-nitrobenzylcysteine containing peptide. The peak 16 Da less than [3NB+H]+ is loss of hydroxyl radical from the charge reduced cation radical, [3NB+2H]+• based on our discussion. a) and b) are magnified Figures 1f and 3f, respectively, in m/z region between 2050 and 2140 around [3NB+2H]+• ion. c) was recorded using a time of flight mass spectrometer equipped with a 337nm N2 laser in the reflector mode. 100 shots were averaged. 10 mg/ml CHCA was used for matrix.