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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Chem Res Toxicol. 2009 Mar;22(3):574–583. doi: 10.1021/tx8003449

Figure 2.

Figure 2

Primer extension assays for nucleotide incorporation opposite tandem lesions, i.e., 5′-(8-oxodG)-Tg-3′ and 5′-Tg-(8-oxodG)-3′, a single 8-oxodG or Tg, and the undamaged control, with exo- Klenow fragment (a) and yeast pol η (b). 5′-[32P]-labeled d(GCTAGGATCATAGC) was used as the primer. Klenow fragment or yeast pol η at the indicated units/concentrations was incubated with 10 nM substrate and 200 μM dNTPs at 37 °C for 60 min. The products were subsequently resolved by using 20% denaturing polyacrylamide gels. The 21-mer was observed due to the presence of a 1-base overhang in the primer, and the 22-mer primer extension products were originated from the terminal transferase activity of the polymerase.