Table 3.

Steady-state kinetic parameters for nucleotide incorporation mediated by yeast pol η opposite Tg and 8-oxodG in tandem lesion-containing substrates and opposite undamaged dG and dT in the control substrate^a

Substrates	dNTP	kcat (min-1)	km (nM)	kcat / Km (nM-1 min-1)	finc
5′-Tg-(8-oxodG)-3′	14 mer Primer: 5′ - GCTAGGATCATAGC - 3′
	dATP	0.29 ± 0.02	(2.3 ± 0.3) × 103	1.3 × 10-4	4.5 × 10-2
	dGTP	0.060 ± 0.005	(4.9 ± 0.9) × 103	1.2 × 10-5	4.1 × 10-3
	dCTP	0.099 ± 0.029	34 ± 16	2.9 × 10-3	1.0
	dTTP	0.060 ± 0.005	(5.8 ± 0.7) × 103	1.0 × 10-5	3.4 × 10-3
	15 mer Primer: 5′ - GCTAGGATCATAGCC - 3′
	dATP	0.063 ± 0.001	(2.9 ± 0.7) × 102	2.2 × 10-4	1.0
	dGTP	0.16 ± 0.01	(6.3 ± 0.4) × 104	2.5 × 10-6	1.1 × 10-2
	dCTP	0.11 ± 0.002	(6.3 ± 0.5) × 105	1.7 × 10-7	7.7 × 10-4
	dTTP	0.13 ± 0.01	(2.7 ± 0.7) × 105	4.8 × 10-6	2.2 × 10-3
5′-(8-oxodG)-Tg-3′	14 mer Primer: 5′ - GCTAGGATCATAGC - 3′
	dATP	0.12 ± 0.002	(3.3 ± 0.1) × 102	3.6 × 10-4	1.0
	dGTP	0.048 ± 0.002	(1.4 ± 0.1) × 104	3.4 × 10-6	9.4 × 10-3
	dCTP	0.063 ± 0.005	(2.5 ± 0.4) × 103	2.5 × 10-5	6.9 × 10-2
	dTTP	0.080 ± 0.002	(5.3 ± 0.5) × 104	1.5 × 10-6	4.2 × 10-3
	15 mer Primer: 5′ - GCTAGGATCATAGCA - 3′
	dATP	0.084 ± 0.002	(1.1 ± 0.1) × 105	7.6 × 10-7	6.9 × 10-3
	dGTP	0.15 ± 0.01	(9.6 ± 0.5) × 105	1.6 × 10-7	1.5 × 10-3
	dCTP	0.046 ± 0.005	(4.1 ± 0.6) × 102	1.1 × 10-4	1.0
	dTTP	0.17 ± 0.02	(6.4 ± 0.5) × 105	2.7 × 10-7	2.5 × 10-3
5′-dG-dT-3′	14 mer Primer: 5′ - GCTAGGATCATAGC - 3′
	dATP	0.055 ± 0.005	36 ± 1	1.5 × 10-3	1.0
	dGTP	0.080 ± 0.005	(5.9 ± 0.5) × 104	1.4 × 10-6	9.3 × 10-4
	dCTP	0.077 ± 0.010	(4.1 ± 0.1) × 103	1.9 × 10-5	1.3 × 10-2
	dTTP	0.13 ± 0.003	(1.1 ± 0.9) × 105	1.2 × 10-6	8.0 × 10-4
	15 mer Primer: 5′ - GCTAGGATCATAGCA - 3′
	dATP	0.23 ± 0.02	(5.2 ± 0.3) × 105	4.4 × 10-7	4.6 × 10-5
	dGTP	0.38 ± 0.01	(5.8 ± 0.07) × 105	6.7 × 10-7	7.1 × 10-5
	dCTP	0.087 ± 0.010	9.2 ± 2.5	9.5 × 10-3	1.0
	dTTP	0.087 ± 0.010	(1.8 ± 0.3) × 104	4.8 × 10-6	5.1 × 10-4

^ak_cat and K_m are average values based on three independent measurements