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Ligation experiment of gapped pGLuc1temGG (left) and pGLuc2temGTG (right) in presence of different insertion strands with T4 DNA Ligase at 16°C for 12 h; lane 1: gapped pGLuc1temGG alone, lane 2: plasmid + 13-is, lane 3: plasmid + 13-is-Pt, lane 4: plasmid + 13-is-oxPt; lane 5: gapped pGLuc2temGTG alone, lane 6: plasmid + 27-is, lane 7: plasmid + 27-is-Pt, lane 8: plasmid + 27-is-oxPt.
