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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Nat Prod. 2009 Mar;72(3):469–472. doi: 10.1021/np800598t

Figure 2.

Figure 2

FTMS monitoring of the loading of benzoate and salicylate on the ACP EncC. A. ATP (10 mM)-dependent transfer of benzoic acid (10 mM) by EncN (2 µM) on holo-EncC (20 µM); B. Sfp-catalyzed phosphopanthetheinylation of apo-EncC (20 µM) by benzoyl-CoA7 (~0.25 mM) generated in situ; C. Same reaction conditions as panel A except that equimolar amounts of benzoic, salicylic, and 2,3-dihydroxybenzoic acids were added; and D. Same reaction conditions as panel A except that benzoic acid was omitted.