Figure 2.
1H-15N HSQC spectra of p53(13-61) and KIX. (a) 1H-15N HSQC spectra of [15N]p53(13-61) free (black) and in the presence of 2 fold excess unlabeled KIX (red). (b) 1H-15N HSQC spectra of [15N]KIX free (black) and in the presence of 2 fold excess unlabeled p53(13-61) (red). All residues are labeled on the bound (1:2 mole ratio) peaks. Cross peaks from the two tryptophan side-chains of p53 are shown in the inset.