Figure 3.

Secondary ¹³Cα chemical shifts and weighted average chemical shift changes of p53(13-61). (a) ¹³Cα shifts calculated by subtraction of published random coil values from the experimental ¹³Cα chemical shifts for free p53(13-16) (black dots) and KIX bound p53(13-16) (red dots). (b) Histogram showing weighted average chemical shift changes $\Delta \delta (\mathrm{N},\mathrm{H}{)}_{\mathrm{av}}(=\sqrt{}\left[\right(\Delta {\delta }_{\mathrm{HN}}{)}^2+(\Delta {\delta }_{\mathrm{N}}∕5{)}^2]$, where Δδ_HN and Δδ_N correspond to the differences in amide ¹H and ¹⁵N chemical shifts between the free and bound states) for p53 amide resonances caused by binding to KIX.