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. Author manuscript; available in PMC: 2010 Mar 3.
Published in final edited form as: Biochemistry. 2009 Mar 3;48(8):1838–1846. doi: 10.1021/bi8020789

Figure 2.

Figure 2

PRL1 activates Src and ERK1/2. (A) Cell lysates (50 μg) were resolved by SDS-PAGE and subjected to Western blotting to detect pSrc527, pSrc416, and Src. Actin was used as a loading control. Kinase activity of the immunoprecipitated Src was measured with enolase and [32P]ATP as substrates. (B) Activation status of p130Cas, FAK, and ERK1/2. p130Cas and FAK were immunoprecipitated from cell lysates (1000 μg) and resolved by SDS-PAGE. Tyrosine phosphorylation levels were detected by Western blot using anti-pY20 antibody. ERK1/2 and pERK1/2 were detected by Western blot from total cell lysates (50 μg).