Table 3.
Formation of tetraols (9 plus 12) from Phe-1,2-diol (7) and Phe-3,4-diol (8)a
| pmol 9 + 12 per incubation |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Human hepatocyte sample and incubation time (h) | ||||||||||
| Substrate | Substrate Concentration |
1 | 2 | 3 | ||||||
| µM | 6 | 12 | 24 | 6 | 12 | 24 | 6 | 12 | 24 | |
| Phe-1,2-diol (7) | 1 | 9.5 | 13.0 | 18.5 | 6.3 | 8.2 | 10.9 | 10.7 | 16.8 | 21.8 |
| 10 | 32.4 | 45.9 | 59.8 | 57.4 | 73.5 | 86.5 | 62.2 | 105 | 135 | |
| Phe-3,4-diol (8) | 1 | 23.3 | 49.1 | 131 | 23.1 | 48.8 | 128 | 31.8 | 87.4 | 296 |
| 10 | 97.3 | 239.8 | 853 | 159 | 546 | 2490 | 158 | 665 | 3240 | |
a
Human hepatocytes were incubated with racemic 7 or 8 (1 or 10 µM) for 6, 12, or 24h in a total volume of 2 ml. Tetraols 9 and 12 were analyzed together as a racemic mixture.