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. Author manuscript; available in PMC: 2010 May 20.
Published in final edited form as: Bioconjug Chem. 2009 May 20;20(5):937–943. doi: 10.1021/bc800520d

Figure 2.

Figure 2

In vitro uptake of Cy5.5 labeled synthetic HDL agents in J774A.1 murine macrophages. Uptake was assessed using an IVIS 200 fluorescent imaging system. A) Fluorescent images of cells treated with 18A-Gd or 37pA-Gd, with 18A-Gd or 37pA-Gd and a tenfold excess of agent without Cy5.5, untreated cells or treated with Gd-micelles. B) Quantified fluorescence of macrophages compared with macrophages under competition-inhibition conditions and untreated macrophages. Incubation conditions were 2 hours and 0.02 mM (by Gd) of agent. * indicates p<0.01 as compared to the treated macrophage cells. C) Comparison of the uptake of 18A-Gd and 37pA-Gd in macrophages and smooth muscle cells. D) and E) show the quantified fluorescence of 18A-Gd and 37pA-Gd respectively, when incubated for 2 hours at the indicated concentrations (the solid lines are logarithmic lines of best fit generated by a Matlab program). Error bars are equal to one standard deviation and n=12 in all cases.

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