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. Author manuscript; available in PMC: 2010 May 26.
Published in final edited form as: ACS Nano. 2009 May 26;3(5):1203–1211. doi: 10.1021/nn9000148

Figure 2.

Figure 2

Schematic diagram of AFM probe functionalized with Tf interacting with positively charged CNPs, the force-extension spectrum and the force, length histograms obtained on CNPs with different ZPs. For protein-CNP interaction studies, (a) succinimide functionalized AFM tip was bioconjugated with Tf to form (b) Tf terminated AFM probe. (c) Interaction of Tf terminated AFM tip with protonated CNPs. Force-extension plots of Tf interacting with CNPs of ZP (d) +36.4±0.7 mV; (e) +5.9±1.2 mV; and (f) -35.1±0.9 mV. The corresponding force (g, i, and k) and length histograms (h, j, and l) are also shown. Force and length histograms were obtained by conducting multiple SMFS measurements on each sample. The total number of force and length values analyzed were n=273 (g and h), 206 (i and j) and 210 (k and l).