Table 1.
Protein biomarkers selected as candidates for multiplexed microsphere-based array development.
| Cytokine Panel | ||||||
|---|---|---|---|---|---|---|
| Analyte | Common Name | UNIGENE Number | Molecular Weight (kDa) | Array Limit of Detection (LOD) (pM) | Coefficient of Variation Range (CV, in %) | ELISA Limit of Detection (LOD) (pM) |
| VEGF | Vascular endothelial growth factor | Hs.73793 | 42 | 469 | 7 − 60 | 0.119 |
| EGF | Epidermal growth factor | Hs.419815 | 6 | 11 | 10 − 72 | 0.117 |
| IP-10 | Interferon-inducible protein, 10 kDa (CXCL10) | Hs.632586 | 10 | 140 | 13 − 66 | 0.167 |
| IL-8 | Interleukin-8 (CXCL8) | Hs.624 | 8 | 8 | 8 − 68 | 0.438 |
| MCP-1 | Monocyte chemoattractant protein-1 (CCL2) | Hs.303649 | 8.7 | 90 | 10 − 64 | 0.574 |
| TIMP-1 | Tissue inhibitor of matrix metalloproteinase-1 | Hs.522632 | 28 | 167 | 19 − 57 | 2.86 |
| RANTES | Regulated upon activation, normal T cell expressed and secreted (CCL5) | Hs.514821 | 8 | 10 | 7 − 65 | 0.250 |
| MIP-1β | Macrophage inflammatory protein-β (CCL4) | Hs.75703 | 7.8 | 19 | 5 − 65 | 0.513 |
| Eotaxin-2 | MPIF-2, CCL24 | Hs.247838 | 10.6 | 15 | 5 − 80 | 0.236 |
| IL-6 | Interleukin-6 | Hs.654458 | 21 | 64 | 9 − 61 | 0.033 |