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. Author manuscript; available in PMC: 2009 Oct 22.
Published in final edited form as: J Neurophysiol. 2008 Apr 16;99(6):2929–2937. doi: 10.1152/jn.00066.2008

Figure 5. TRPM5-GFP+ cells from the anterior nasal cavity respond specifically to denatonium benzoate through a PLC-mediated pathway.

Figure 5

A. TRPM5-GFP+ cells respond with a rapid increase in intracellular calcium to 15 mM denatonium (den) but not to the trigeminal stimulants menthol (men) (0.1 mM) and caffeine (caff) (50 mM) (although this cell gave an off response to caff). Denatonium responses in TRPM5-GFP+ cells are completely blocked by the PLC inhibitor U73122 (2 μM). B. TRPM5-GFP+ cells respond to denatonium (den) but not to the bitter compounds salicin (sal) (15 mM) or cycloheximide (cyx) (1 mM). TRPM5-GFP+ cells also do not respond to capsaicin (caps) (10 μM). C. Dose response curve for denatonium in TRPM5-GFP+ cells. For each point n=5 and error bars show SEM. D. The detection of denatonium is specific to TRPM5-GFP+ cells as adjacent non-GFP epithelial cells do not respond.