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. 2009 Oct;158(3):713–725. doi: 10.1111/j.1476-5381.2009.00323.x

Figure 3.

Figure 3

Effects of GB10 on expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in microglial cells induced by interferon-γ (IFN-γ). (A) Western blots for iNOS, COX-2 and β-actin proteins from microglial cells. (B) Relative density of iNOS immunoblot. (C) Relative density of COX-2 immunoblot. The relative intensity of the IFN-γ-stimulated group was taken to be 100%. Band intensities were quantified by densitometry and are indicated as a percentage change relative to that of the IFN-γ-stimulated group. Western blotting with β-actin was performed to verify that equivalent amounts of protein were loaded in each lane. The experiment was repeated five times. *, significantly different from the IFN-γ-stimulated group (P < 0.05).