Figure 1.

SAHA inhibits proliferation and causes accumulation of acetylated histones in T24 cells. (A) SAHA time course and dose response. Cells were harvested and cell number and viability determined as indicated. Each time point was performed in triplicate, and data are presented as means. (B) T24 cells were cultured with the indicated concentrations of SAHA for 24 h. Cells were harvested and DNA content was assessed by FACS analysis of propidium iodide-stained nuclei. (C) Western blot analysis of acetylated histones H3 and H4 in T24 cells. Histones were isolated by acid extraction from cells cultured for indicated times with 7.5 μM SAHA. Acetylation was detected by using antiacetylated H3 and H4 antibodies. A parallel gel was stained with Coomassie blue for a loading control.