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. 2000 Aug 22;97(18):10014–10019. doi: 10.1073/pnas.180316197

Figure 3.

Figure 3

SAHA induces accumulation of acetylated histone H4 in chromatin associated with the p21WAF1 gene. (A) Schematic representation of the human p21WAF1 gene. Primer sets are indicated as P3, P2, P1, I1, and E2. Sp1 sites are at −82 and −69. (B) Soluble chromatin was immunoprecipitated with antiacetylated histone H4 antibodies from T24 cells cultured without (odd-numbered lanes) and with (even-numbered lanes) 7.5 μM SAHA for 2 h. PCR primers for the regions of the p21WAF1 gene as indicated above were used to amplify the DNA isolated from the immunoprecipitated chromatin. (C) The figure in B was scanned and quantified by using nih image 1.62 analysis software. The ratio between input DNA and precipitated DNA was calculated for each treatment and primer set. The fold increase after treatment with SAHA was calculated from the indicated ratios.