Figure 5.
SAHA does not induce accumulation of acetylated histone H4 in the chromatin associated with actin and p27 genes. (A) Northern blot analysis of p27 mRNA expression. Total RNA (10 μg) was isolated and fractionated on 1.2% agarose and transferred to a nylon membrane. The membrane was probed by using 32P-random labeled human p27 cDNA, the γ-actin cDNA, and a 50-mer oligonucleotide specific for 18S rRNA. (B) Soluble chromatin was immunoprecipitated with antiacetylated H4 antibodies from T24 cells cultured without and with 7.5 μM SAHA for 2 h. PCR primers for the regions of the actin and p27 genes as indicated in Materials and Methods were used to amplify the regions from the acetylated chromatin.