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Immunofluorescent identification of rat aortic endothelial cells. Cells cultured on coverslips were incubated with rabbit anti-rat factor VIII (a stable endothelial antigen) antibody and then incubated with Cy-3 conjugated secondary antibody; the nuclei were stained with DAPI. In the negative control, factor VIII antibody was omitted (magnification: 200×).
