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A summary of the RT-PCR primer sequences used to amplify GAPDH, COX-2 and ICAM-1
| Sequences | Product size (bp) | Annealing T (C) | ||
|---|---|---|---|---|
| GAPDH | Sense | TCCCAGAGCTGAACGGGAAGCTCACTG | 339 | 68.1 |
| Antisense | TGGAGGCCATGTAGGCCATGAGGTCCA | |||
| COX-2 | Sense | TTCACCAGACAGATTGCTGGC | 530 | 63.5 |
| Antisense | AGTCTGGAGTGGGAGGCACTTG | |||
| ICAM-1 | Sense | AGAAGGACTGCTTGGGGAA | 332 | 58.1 |
| Antisense | CCTCTGGCGGTAATAGGTG | |||
