Figure 2.

The effect of fisetin on β-hexosaminidase release from LAD 2 cells stimulated by IgE cross-linking. Cells were incubated overnight with 1 µg·mL⁻¹ chimeric human IgE anti-NP (4-hydroxy-3-nitrophenylacetate). IgE sensitized cells were incubated with or without fisetin for 15 min; 100 ng·mL⁻¹ of the antigen (NP-bovine serum albumin) was then added and the cells incubated for 30 min. The rate of β-hexosaminidase release was determined using an enzymatic assay. Data are expressed as mean ± standard error from three different experiments. *P < 0.05, **P < 0.01, significant difference from LAD 2 cells alone; and ##P < 0.01, significant difference from IgE/antigen.