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. 2009 Aug 20;158(3):907–919. doi: 10.1111/j.1476-5381.2009.00365.x

Figure 6.

Figure 6

The effect of fisetin on expression of intercellular adhesion molecule-1 (ICAM-1) and CD40 HMC-1 cells stimulated by activated T cell membranes. HMC-1 cells were pre-incubated with or without the indicated concentration of fisetin for 15 min. Resting (Tc-m) or phorbol-12-myristate 13-acetate-activated T cell membranes (aTc-m) were then added and the cells incubated for a further 16 h. (A,B) Gene expression levels were determined by real-time RT-PCR, normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and plotted relative to those in cells treated with aTc-m alone. Data are expressed as mean ± standard error from three different experiments. **P < 0.01, significant difference from HMC-1 cells alone; and #P < 0.05, ##P < 0.01, significant difference from aTc-m. (C) The level of ICAM-1 protein was determined by Western blot analysis. (D) The level of CD40 protein was determined by a flow cytometer.