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The effect of fisetin on phosphorylation of MAP kinases (MAPKs) [extracellular signal-regulated kinase (ERK)1/2 and p38] in HMC-1 cells. HMC-1 cells were pre-incubated with or without the indicated concentration of fisetin for 15 min. Resting (Tc-m) or phorbol-12-myristate 13-acetate-activated T cell membranes (aTc-m) were added and the cells incubated for a further 20 min. The levels of MAPKs and phosphorylated MAPKs were determined by Western blot analysis.
