Table 4. QR uptake is prevented by anti-ANX antibody.

Inhibition of QR uptake in presence of Anti-annexin XII	Inhibition of QR uptake in presence of anti AP-180	Inhibition of QR uptake in presence of BSA
1,25 µg (BSA 500 ng) +	5 µg (BSA 50 ng) −	5 µg −
500 ng (BSA200 ng) +	2 µg (BSA 20 ng) −	2 µg −
250 ng (BSA100 ng) +	1 µg (BSA 10 ng) −	1 µg −
125 ng (BSA 50 ng) +		500 ng −
25 ng (BSA10 ng) −		50 ng −

Groups of six polyps were equilibrated in SolHy pH 4 in presence of the indicated doses of the affinity purified antibodies (rabbit anti-annexin XII, rabbit anti human endocytic accessory protein AP180) or of bovin serum albumine (BSA). 1 h later QRs were added to the polyps and after at least 4 h of incubation the presence of internalization was monitored under a fluorescence microscope. As BSA is used for IgG stabilization, in each column is reported the amount of antibody used, together with the BSA co-administrated (in brackets). An additional set of experiment was performed by supplying pure BSA at different concentrations, to rule out its aspecific role in the uptake process. Inhibition of QR uptake, indicated by the plus sign (+) was observed in presence of anti-ANX, while was not prevented by either aspefic antibody nor BSA even at higher doses [indicated by the sign minus (−)].