Skip to main content
. 2009 Nov 2;4(11):e7668. doi: 10.1371/journal.pone.0007668

Figure 5. Northern blot analysis of sRNA stability.

Figure 5

Aliquots of mid-exponential or late stationary phase cultures of strain MGAS2221 were harvested prior to (T = 0) and following (T = 5, 10, 20, 30, 45, 60, 90 min) rifampicin treatment to inhibit new RNA synthesis. 8 µg of extracted RNA from each time-point was subjected to Northern blot analysis, probing for PEL, FASX, SR195750, SR914400, SR1251900, SR1719800, and SR1754950 transcripts. Note that as the exposure time of each Northern blot varied no comparison of band intensities between blots should be made.