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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Biochemistry. 2009 Oct 13;48(40):9471–9481. doi: 10.1021/bi901034r

Figure 2.

Figure 2

NBD-CQ fluorescence measured via thin layer calibration methods (see [24]) in the presence of 100 nM heme (○), 1 µM heme (□), or no heme (Δ). Heme solutions were prepared from 5 mM hematin dissolved in 0.1 M NaOH, titrated to pH 5.5, and then added to different concentrations of NBD-CQ dissolved in 50 mM MES-Tris pH 5.5.