. Author manuscript; available in PMC: 2010 Oct 13.

Published in final edited form as: Biochemistry. 2009 Oct 13;48(40):9471–9481. doi: 10.1021/bi901034r

Table 2.

Summary of Accumulation Kinetics at 250 nM NBD-CQ external concentration.

	DV Internal concentration at plateau^a (µM)
Glucose^* VPL^{^}	+ −	−^# −	+ +
HB3	10.6 ± 1.0	8.20 ± 0.94	9.99 ± 0.46
Dd2	7.33 ± 0.72	10.8 ± 0.74	8.95 ± 0.5
C2^GCO3	9.81 ± 0.56	8.26 ± 0.64	10.1 ± 0.76
C4^Dd2	7.91 ± 0.56	10.8 ± 1.0	9.98 ± 0.73
	Initial Rates (µmol L⁻¹sec⁻¹ × 10⁻²)
Glucose^* VPL^{^}	+ −	−^# −	+ +
HB3	4.24 ± 0.47	2.22 ± 0.35	4.94 ± 0.35
Dd2	2.61 ± 0.22	3.23 ± 0.34	3.48 ± 0.27
C2^GCO3	3.90 ± 0.32	1.56 ± 0.22	4.69 ± 0.51
C4^Dd2	2.37 ± 0.17	2.55 ± 0.44	3.61 ± 0.32
	Rate Constants (sec ⁻¹ × 10⁻³)
Glucose^* VPL^{^}	+ −	−^# −	+ +
HB3	8.03 ± 0.63	4.43 ± 0.25	9.85 ± 0.61
Dd2	5.29 ± 0.53	4.63 ± 0.39	7.21 ± 0.76
C2^GCO3	7.05 ± 0.33	3.42 ± 0.37	9.43 ± 0.65
C4^Dd2	4.24 ± 0.32	3.91 ± 0.30	8.51 ± 0.83

5mM D-Glucose

No glucose substitution

^{^}

1µM Verapamil

Using external 250 nM NBD-CQ, influx kinetics were measured in the presence (2^nd column), vs absence of glucose (3^rd column) and with a constant external concentration of 1 µM Verapamil (4^th column) for several parasite lines; CQS parasites HB3 and C2^GCO3, and CQR parasites Dd2 and C4^Dd2. Similar patterns were also measured at 100 and 500 nM NBD-CQ in the perfusate (data not shown).