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. Author manuscript; available in PMC: 2009 Oct 22.
Published in final edited form as: Cell Microbiol. 2008 Apr 28;10(9):1787–1800. doi: 10.1111/j.1462-5822.2008.01165.x

Figure 1. Over-expression of IGTP induces up-regulation of p-FAK (Tyr397, Tyr925) and p-Paxillin (Tyr-118).

Figure 1

IGTP expression was induced in Tet-On/IGTP HeLa cells with Dox at 1 μg/ml, and whole cell lysates were prepared at indicated time points and subjected to immunoblot analysis using indicated antibodies. The membrane was then stripped and probed for detection of total FAK, which was used as the equal loading control. Results were quantitated by densitometric analyses of the bands and normalized by the relative ratio of p-FAK or p-Paxillin to total FAK. The relative ratio at time 0 h is arbitrarily set to 1.0. Fold increase is the ratio of the amount of each time point to that of time 0 h. These data are representative of two independent experiments.