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. Author manuscript; available in PMC: 2009 Oct 22.
Published in final edited form as: Cell Microbiol. 2008 Apr 28;10(9):1787–1800. doi: 10.1111/j.1462-5822.2008.01165.x

Figure 2. FRNK overexpression inhibits IGTP-induced upregulation of p-Akt.

Figure 2

A. Tet-On/IGTP HeLa cells were transfected with 2 μg/ml FRNK-encoding plasmid and then induced with 1 μg/ml Dox 16 h post transfection, Cell morphology was observed under a phase-contrast microscope 48 h pi. B. Tet-On/IGTP HeLa cells at 80% confluence were transfected with 1 μg/ml FRNK using lipofectamine 2000 and then induced with 1 μg/ml Dox 16 h post transfection. Cell lysates were harvested 12 h post induction and subjected to immunoblot analysis with indicated antibodies, where β-actin was used as the loading control. C. Results were quantitated by densitometric analyses and normalized as the relative ratio of p-FAK, p-Paxillin or p-Akt to β-actin. The experiment was repeated twice.