CD24 negatively regulates the immune response to AAP-induced liver injury. CD24-/- mice or WT mice were treated with AAP (10 mg/mouse, dissolved in H20) or vehicle control. (A) Survival of mice 20 hours after treatment. Numbers on graph indicate the number of viable over total mice used per group. All WT mice remained healthy. (B) Serum levels of IL-6, MCP-1 and TNF-α at 6 hours after AAP injection (mean ± SD, n = 5; *P < 0.02, **P < 0.009; ***P < 0.002, student’s t-test). (C) ALT levels measured at 6 hours after treatment (mean ± SD, n = 5; ***P < 0.00004, student’s t-test). Data shown in (B) and (C) have been repeated 2 times. (D) Livers were isolated at 9 hours after treatment. Representative images (20x) of H&E staining are shown (n = 3).