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. 2008 Sep 6;95(2):117–124. doi: 10.1159/000153095

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Copyright © 2008 by S. Karger AG, Basel

This is an open access article distributed under the terms of Karger's Author's Choice™ licensing agreement, adapted from the Creative Commons Attribution Non-Commercial 2.5 license. This license allows authors to re-use their articles for educational and research purposes as long as the author and the journal are fully acknowledged.

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Fig. 2 — Mature and pro-SP-B peptides from trachéal aspirate, amniotic fluid, and normal adult bronchoalveolar lavage (BAL) under nonreducing conditions. Immunoblots of TA samples from 3 newborn infants with RDS (RDS 1, 3, 4), 3 control infants (CON 1, 2, 3), 8 amniotic fluid samples (AF 1-8), 6 BAL specimens from adults (BAL 1-6), purified human SP-B (hSP-B), and Infasurf were probed with antibody to mature or pro-SP-B. Locations of common mature and pro-SP-B peptides (M_r 42, 40, 24, and 21 kDa) are indicated. TA samples from RDS 1 obtained 1 week apart (TA1 vs. TA2) demonstrate changes in mature and pro-SP-B peptide mobilities over time in the same infant. Antibody to pro-SP-B does not detect any pro-SP-B peptides in purified human SP-B or in Infasurf.